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By C. L. Tsou

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London 33, 159 (1976). U . S . Liang, D. Yang, and L. Zhang, Sei. , Ser. Β (Engl. ) 30, 149 (1987). 12. H. Kiltz, W. Keil, Μ. Griesbach, Κ. Petry, and Η. Meyer, Hoppe-Seyler's Ζ. Physiol. Chem. 358, 123 (1977). 13. S. Liang and L. Zhang, Sei. , Ser. Β (Engl. ) 30, 270 (1987). 14. D. H. Wurster-Hill and M. Bush, Cytogenet. Cell Genet. 27, 147 (1980). 15. S. J. O'Brien, W. G. Nash, D. E. Wildt, Μ. E. Bush, and R. E. Benveniste, Nature (London) 317, 140 (1985). 16. C. S. Craik, C. Largman, T. Fletcher, S.

F u r t h e r m o r e , [P] = ^i- A[Y] = m (k K +0 m ( + Ä:' [S])[Y] 7) y +0 * w h e r e V = k [E] . A plot of 1/[PL against 1/[S] gives a straight line with a slope of k K [Y]/V and an ordinate intercept of k'+ [Y]/V. Since K , [Y], and V are k n o w n quantities, the microscopic rate c o n s t a n t s , k and k' can then be determined. +2 +0 0 m 0 m +0 +0 B. Complexing Modifiers F o r modifiers complexing reversibly with the e n z y m e to form E Y before the irreversible modification step leading to E Y ' , it can be s h o w n that the a p p a r e n t inhibition rate constant is (8) 54 Tsou F r o m a c o m p a r i s o n of the respective equations it is clear that 1/A is inde­ p e n d e n t of [Y] for noncomplexing inhibition but a plot of 1/A against [Y] gives a straight line with a positive slope for complexing inhibition.

H. -F. -X. Zhu, Acta Zool. Sin. 22, 137 (1976). 6. -H. -P. -Y. Tian, Kexue Tongbao (For. Lang. ) 30, 1396 (1985). 7. Trichosanthin Group, Kexue Tongbao (Chin. ) 23, 176 (1978). 8. -Z. , Sei. , Ser. B. (Engl. ) 25, 730 (1982). 9. -Z. , Sei. , Ser. B. (Engl. ) 29, 26 (1986). 10. -Z. , Sei. , Ser. Β (Engl. ) 30, 386 (1987). 11. A. Jack and M. , Sect. A A34, 931 (1978). 12. S. -J. -H. -C. -Z. Chen, Kexue Tongbao (For. Lang. ) 33, 1743 (1988). 13. T. A. Steitz, R. J. Fletlerick, W. F. Audersobn, and C.

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