By C. L. Tsou
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Extra resources for Current Biochemical Research in China
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F u r t h e r m o r e , [P] = ^i- A[Y] = m (k K +0 m ( + Ä:' [S])[Y] 7) y +0 * w h e r e V = k [E] . A plot of 1/[PL against 1/[S] gives a straight line with a slope of k K [Y]/V and an ordinate intercept of k'+ [Y]/V. Since K , [Y], and V are k n o w n quantities, the microscopic rate c o n s t a n t s , k and k' can then be determined. +2 +0 0 m 0 m +0 +0 B. Complexing Modifiers F o r modifiers complexing reversibly with the e n z y m e to form E Y before the irreversible modification step leading to E Y ' , it can be s h o w n that the a p p a r e n t inhibition rate constant is (8) 54 Tsou F r o m a c o m p a r i s o n of the respective equations it is clear that 1/A is inde p e n d e n t of [Y] for noncomplexing inhibition but a plot of 1/A against [Y] gives a straight line with a positive slope for complexing inhibition.
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