By F. Hadziselimovic
Cytological innovations have vastly more desirable within the final 20 years, mostly due to extra improvement of the microscope. Electron microscopy, specifically, has unfolded nice clients for the learn of mobilephone morphology, whereas the enhance ment of radio-immuno-assay has introduced nice growth in endocrinology. The applica tion of those ideas, that are complementary to one another and supply collectively aiding facts, is the topic of this monograph. The paintings is split into elements. the 1st offers with the ultrastructure of standard testicular improvement, describing 4 major parts of the testicle - the germ cells, the Sertoli cells, the peri tubular connective tissue and the Leydig mobilephone- with info in their person improvement. To make the electron micrographs extra simply comprehensible, diagrams were used to provide an explanation for an important issues. the second one half offers with cryptorchid testicles, together with the first and sec ondary adjustments concerned and morphometric stories of the secondary adjustments. the importance of those ultrastructural observations for the remedy of cryptorchidism is emphasised. using radio-immuno-assay the extent of testosterone in cryp torchid mice was resolute and comparisons drawn among the ultrastructural adjustments in cryptorchidism in mouse and guy. The experimental experiences served as a foundation for the speculation that, almost certainly, a congenital disturbance of the hy pothalamo-hypophyso-gonadal axis is chargeable for cryptorchidism. i am hoping that this monograph will give a contribution in the direction of a greater realizing of standard and cryptorchid testicular improvement and of the etiology of cryptorchidism.
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Extra info for Cryptorchidism: Ultrastructure of Normal and Cryptorchid Testis Development
There is no significant difference between the mean testis weight of control mice (129 g) and that of E2 B mice (126 g). Discussion The most widely held theory of. testicular development from birth to puberty was that ofCharny et al. (1952), who distinguished three phases in the development of the testicle: the static phase, the growth phase and the maturation phase. Stadtler (1975) rejected the theory of development in phases, on the grounds that the seminiferous tubule showed a continuous increase in diameter and in the number of spermatogonia up to the eleventh year.
The Sb-type, on the other hand, is much rarer and the isolated cells seem to have less smooth endoplasmic reticulum and lipoid droplets than those in normal testicles from the same agegroup. Morphometric assessment of the Sertoli cells up to the age of six revealed no significant difference in number or in individual cell volume. 617 x 10 6 • The single cell volume in normal testicles is 491 cu and in undescended testicles 429 cu. The differences in both the number of Sertoli cells and in single cell volume are not Significant (Table 2).
The mitochondria are round to longish in shape and of the crista type. They are to be found almost equally frequently in the basal and apical parts of the cell. The micropinocytotic vesicles are concentrated in the basal part of the cell membrane. The rough endoplasmic reticulum is situated mainly in the apical part, arranged in three to six rows aligned towards the point of the cell. In the Sb-type of Sertoli cell, smooth endoplasmic reticulum is again found in abundance. With increasing age, the endoplasmic reticulum becomes more differentiate.