Download Chemical Proteomics: Methods and Protocols (Methods in by Gerard Drewes, Marcus Bantscheff PDF

By Gerard Drewes, Marcus Bantscheff

The multidisciplinary technological know-how of chemical proteomics reviews how small molecules of man-made or common beginning bind to proteins and modulate their functionality.  In Chemical Proteomics: equipment and Protocols, professional researchers within the box supply key thoughts to enquire chemical proteomics targeting analytical options, how probes are generated, suggestions for the invention of small molecule ambitions and the probing of aim functionality, and small molecule ligand and drug discovery.  Written within the hugely profitable equipment in Molecular Biology™ sequence structure, chapters comprise introductions to their respective themes, lists of the mandatory fabrics and reagents, step by step, without problems reproducible laboratory protocols, and key pointers on troubleshooting and warding off identified pitfalls.  Authoritative and functional, Chemical Proteomics : equipment and Protocols seeks to supply methodologies that might give a contribution to a much wider software of chemical proteomics equipment in biochemical and phone organic laboratories.

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Microwave oven. 10. SimplyBlue Safestain (Invitrogen, Carlsbad, CA). 11. Semidry Trans-blot transfer apparatus (BioRad, Hercules, CA). 12. Transfer buffer concentrate (NuPAGE, Invitrogen, Carlsbad, CA). For two gels, use 80 mL ultra pure water, 10 mL methanol, and 10 mL transfer buffer concentrate. 13. Extra thick filter pads (BioRad, Hercules, CA). 14. Nitrocellulose membranes (NuPAGE, Invitrogen, Carlsbad, CA). 15. Protein detector Western blot Kit (KPL, Gaithersburg, MD). 16. Polyclonal anti-PKC-α antibody (Cell Signaling Technology, Beverly, MA).

Tallarico, J. , Porter, J. , and Cong, F. (2009) Tankyrase inhibition stabilizes axin and antagonizes Wnt signalling. Nature 461, 614–620. 11. Fleischer, T. , Murphy, B. , Flick, J. , Terry-Lorenzo, R. , Gao, Z. , Willardsen, J. , and Boniface, J. J. (2010) Chemical proteomics identifies Nampt as the target of CB30865, an orphan cytotoxic compound. Chem. Biol. 17, 659–664. 12. , Jeffrey, K. , Chung, C. , Rice, C. , Lora, J. , Prinjha, R. , and Tarakhovsky, A. (2010) Suppression of inflammation by a synthetic histone mimic.

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