Urology

Download Atlas of Clinical Andrology by Elsayed S. E. Hafez, Saad Dean Hafez PDF

By Elsayed S. E. Hafez, Saad Dean Hafez

Andrology, the technology of the body structure, pathology and molecular biology of male copy, is a comparatively new area of expertise that has develop into more and more very important with the arrival of contemporary assisted reproductive ideas (ART). The Atlas of medical Andrology describes either general and irregular reproductive body structure, and summarizes fresh examine within the fields of endocrinology, immunoandrology, molecular andrology, and paintings. concentrating on the leading edge of contemporary wisdom and advances in scientific andrology, it comprises the newest scientific examine findings and makes them obtainable to clinicians who desire this crucial details for the best program of assisted reproductive ideas

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Most men with CBAVD have mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) gene, with an increased risk of carrying the 5T variant. Sperm chromosome aneuploidy/globozoospermia Globozoospermia (round-headed sperm) is a very rare condition observed in < 1% of infertility patients. The sperm lack an acrosomal cap. Pregnancy is rare even with application of ICSI. It would 44 appear that other abnormalities may coexist with the lack of an acrosome. An increased frequency of sperm chromosome aneuploidy might be associated with globozoospermia and, therefore, might be responsible for the poor pregnancy prognosis.

DNA integrity is determined by the modified alkaline single-cell gel electrophoresis (Comet) assay under yellow light to prevent further induced DNA damage. 0% normal melting point agarose in Ca2+- and Mg2+-free phosphatebuffered saline covered with a large coverslip. The slides are placed on a chilled metal tray and left at 4°C for ≥ 30 min to allow the agarose to solidify. The coverslips are removed and the slides immersed in a Coplin jar containing freshly prepared cold lysing solution. Testicular sperm from infertile men have more DNA fragmentation when incubated overnight after thawing than those assessed immediately after thawing.

The central core of the middle piece together with the entire length of the tail constitutes the axoneme. The axoneme itself is composed of nine pairs of microtubules that are arranged radially around two central filaments. In the middle piece this 9 + 2 arrangement of microtubules is surrounded by the nine coarse or dense outer fibers that appear to be associated with the nine double fibers of the axoneme. The axoneme and associated dense fibers of the middle piece are covered peripherally by numerous mitochondria.

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