By John A. Sturman, Derek A. Applegarth (auth.), Alan A. Boulton, Glen B. Baker, James D. Wood (eds.)
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Separations of some ammo acids are incomplete, especially n-t the mitral part of the chromatogram. With the rapid advancement of mrcrobore and reverse phase technology m HPLC, the escalating popularity of this analytical technique IS not unexpected. Generally, in HPLC no cleanup procedure is necessary for biological samples. Some of the ammo acids can be analyzed directly without prior denvatization, as is the case with tyrosine, tryptophan, and their metabohtes. However, the use of fluorogenic derrvatlzatron reagents 1s a common practice.
And Geiger J W (1969) Automatrc analysrs of ammo acids. Effect of resin cross-lmkmg and operatronal variables on isolation Anal. Blochem 29, 265-283. , and GJessmg L (1982) Homocarnosinosrs. Hypercarnosmurra I Neurochem. 38, 242-245. Malloy M. H , Rassm D. , and Gaul1 G. E (1981a) A method for the measurement of free and bound plasma cyst(e Anal Bzochem 113, 407415 Malloy M. , and Gaul1 G. E (1981b) Plasma cyst(e m homocyst(e)memra. Am J Clan Nutr 34, 2619-2621. , Laberge C , and Poter M (1983) Deficiency of Fumarylacetoacetase u-r the Acute Form of Hereditary Tyrosmemla Wrth Reference to Prenatal Diagnosis, in Pedlafrx Lzver Dzsease (Fisher M M.
This procedure appears to be sensmve and specific for Tau. SOCl2 -I-BuOCOCl H2NCH2CH2S03H e A-BuOCONHCH2CH2S03 NaOH -I-BuOCONHCH2CH2SO2Cl Fig. 8 Derwatlzatlon nation, and amldatlon. e A-BuOCONHCH2CH2S02NR2 of Tau by lsobutoxycarbonylatlon, chlon- 8. Choice of Chromatographic Column There have been dramatic changes m the GC analysis of ammo acids over the last 20 yr. Earlier chromatographic separations were generally performed on packed columns, usually l-3 m m length, with an inner diameter of 24 mm.